"Burning Man" Showcases Multicolor Flip-out Technique in Drosophila Brain

Neurons labeled using the multicolor flip-out strategy, which stochastically labels small subsets of cells in distinct colors, reveal the organization of the integration center of the Drosophila brain, the central complex, at a single cell level. This technique enabled the identification and detailed morphological characterization of a subset of neurons that arborize throughout this key integration center. This analysis also provided a set of GAL4 lines that identifies subsets of neurons in the neuropils that constitute the central complex. Two neuropils of the central complex are evident in this image, the protocerebral bridge and donut-shaped ellipsoid body.

Tanya Wolff, Nirmala A. Iyer and Gerald M. Rubin.
Neuroarchitecture and neuroanatomy of the Drosophila central complex: A GAL4-based dissection of protocerebral bridge neurons and circuits.
Journal of Comparative Neurology 523, 997-1037 (2015).

IsoView whole-animal functional imaging of larval Drosophila

Lateral (left), dorsoventral (middle) and rotating (right) maximum-intensity projections of a multi-view deconvolved time-lapse recording of an early Drosophila first instar larva expressing the calcium indicator GCaMP6s throughout the nervous system. The data set was acquired with a custom-built light-sheet microscope for isotropic multi-view imaging (IsoView microscope), which provides isotropic, sub-cellular spatial resolution in all three dimensions. Functional imaging was performed at 2 Hz. The images are gamma-corrected and shown using a false-color look-up-table (blue to yellow) to reduce the high dynamic range of the raw data for better visibility.

Raghav Chhetri, Fernando Amat, Yinan Wan, Burkhard Höckendorf, William Lemon and Philipp Keller.
Whole-animal functional and developmental imaging with isotropic spatial resolution.
Nature Methods 12, 1171-8 (2015).