Microcomputed tomography (μ-CT) as a whole fly phenotyping tool
An adult female is shown in 3D and digitally sliced along the XZ and YZ axes to reveal all intact internal structures. Each organ is then sequentially highlighted in color, beginning with anterior structures. The non-destructive nature of the technique permits high resolution imaging at any developmental stage, preserving the spatial architecture of all organs. This makes μ-CT a powerful tool for unbiased characterization of developmental defects and a deeper understanding of inter-organ relationships.
Video Credit: Todd A. Schoborg
Schoborg TA, Smith SL, Smith LN, Morris HD, Rusan NM
Micro-computed tomography as a platform for exploring Drosophila development.
Development. 2019 Dec 11;146(23)
A depth color-coded confocal microscopy image of fruit fly reproductive organs during coitus
The image shows the cuticle of Drosophila genitals in coitus. Genital coupling through interlocking of complementary surfaces is a vital aspect of successful copulation. The genitals (female's on top) were imaged at 400x magnification with a laser scanning confocal microscope and presented as depth color-coded maximum intensity projection after application of 'find edges' filter
Image Credit: Igor Siwanowicz (Drosophila Connectomics Group, Cambridge)
Shao L, Chung P, Wong A, Siwanowicz I, Kent CF, Long X, Heberlein U
A Neural Circuit Encoding the Experience of Copulation in Female Drosophila.
Neuron. 2019 Jun 5;102(5):1025-1036